{"id":203356,"date":"2025-01-08T07:34:08","date_gmt":"2025-01-08T13:34:08","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2025\/01\/fluorescent-rna-complexes-offer-new-insights-into-cellular-dynamics"},"modified":"2025-01-08T07:34:08","modified_gmt":"2025-01-08T13:34:08","slug":"fluorescent-rna-complexes-offer-new-insights-into-cellular-dynamics","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2025\/01\/fluorescent-rna-complexes-offer-new-insights-into-cellular-dynamics","title":{"rendered":"Fluorescent RNA complexes offer new insights into cellular dynamics"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/fluorescent-rna-complexes-offer-new-insights-into-cellular-dynamics.jpg\"><\/a><\/p>\n<p>The specific labeling of RNA in living cells poses many challenges. In a new article <a href=\"https:\/\/www.nature.com\/articles\/s41589-024-01801-3\" target=\"_blank\">published<\/a> in the journal <i>Nature Chemical Biology<\/i>, researchers from the University of Innsbruck describe a structure-guided approach to the formation of covalent (i.e., irreversibly tethered) RNA-ligand complexes.<\/p>\n<p>The key to this is the modification of the original ligand with a reactive \u201chandle\u201d that allows it to react with a nucleobase at the RNA binding site. This was first demonstrated in vitro and in vivo using the example of an RNA riboswitch.<\/p>\n<p>The versatility of the approach is highlighted by the first covalent \u201cfluorescent light-up RNA aptamer\u201d (coFLAP). This system retains its strong fluorescence during imaging in living cells even after washing, can be used for high-resolution microscopy and is particularly suitable for FRAP (fluorescence recovery after photobleaching) for monitoring intracellular RNA dynamics.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The specific labeling of RNA in living cells poses many challenges. In a new article published in the journal Nature Chemical Biology, researchers from the University of Innsbruck describe a structure-guided approach to the formation of covalent (i.e., irreversibly tethered) RNA-ligand complexes. The key to this is the modification of the original ligand with a [\u2026]<\/p>\n","protected":false},"author":396,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"class_list":["post-203356","post","type-post","status-publish","format-standard","hentry","category-futurism"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/203356","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/396"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=203356"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/203356\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=203356"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=203356"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=203356"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}