{"id":201975,"date":"2024-12-19T23:17:36","date_gmt":"2024-12-20T05:17:36","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2024\/12\/addressing-the-puzzle-of-bispecific-antibody-manufacturing"},"modified":"2024-12-19T23:17:36","modified_gmt":"2024-12-20T05:17:36","slug":"addressing-the-puzzle-of-bispecific-antibody-manufacturing","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2024\/12\/addressing-the-puzzle-of-bispecific-antibody-manufacturing","title":{"rendered":"Addressing the Puzzle of Bispecific Antibody Manufacturing"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/addressing-the-puzzle-of-bispecific-antibody-manufacturing.jpg\"><\/a><\/p>\n<p>To help reduce this problem, Evitria says they\u2019ve licensed <a href=\"https:\/\/www.lonza.com\/biologics\/expression-technologies\/bYlok\">Lonza\u2019s bYlok<sup>\u00ae<\/sup> technology<\/a> to improve light chain pairing. The starting point involves antibodies with a \u201cknob\u201d or a \u201chole\u201d in the heavy chains, so-called <a href=\"https:\/\/www.tandfonline.com\/doi\/full\/10.4161\/19420862.2015.989013\">\u201cknobs-into-holes\u201d technology<\/a>, to help improve correct bonding between parts of the bispecific, Schmidt explained.<\/p>\n<p>\u201cWhat you have are two variations, bYlok and non-loked, and another two combinations where one chain has a hole formation and the other a knob formation and vice versa,\u201d he says. \u201cAnd you can express these four combinations to see the impact of the expression level, the yields, <a href=\"https:\/\/www.sciencedirect.com\/topics\/chemistry\/heterodimerization\">heterodimer<\/a> formation, and purity.\u201d<\/p>\n<p>By doing this early screening, he adds, it\u2019s possible for customers to speed up their process development and move to commercial bsAb manufacturing.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>To help reduce this problem, Evitria says they\u2019ve licensed Lonza\u2019s bYlok\u00ae technology to improve light chain pairing. The starting point involves antibodies with a \u201cknob\u201d or a \u201chole\u201d in the heavy chains, so-called \u201cknobs-into-holes\u201d technology, to help improve correct bonding between parts of the bispecific, Schmidt explained. \u201cWhat you have are two variations, bYlok and [\u2026]<\/p>\n","protected":false},"author":662,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"class_list":["post-201975","post","type-post","status-publish","format-standard","hentry","category-futurism"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/201975","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/662"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=201975"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/201975\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=201975"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=201975"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=201975"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}