{"id":195216,"date":"2024-08-25T23:31:07","date_gmt":"2024-08-26T04:31:07","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2024\/08\/a-new-addition-to-the-crispr-toolbox-teaching-the-gene-scissors-to-detect-rna"},"modified":"2024-08-25T23:31:07","modified_gmt":"2024-08-26T04:31:07","slug":"a-new-addition-to-the-crispr-toolbox-teaching-the-gene-scissors-to-detect-rna","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2024\/08\/a-new-addition-to-the-crispr-toolbox-teaching-the-gene-scissors-to-detect-rna","title":{"rendered":"A new addition to the CRISPR toolbox: Teaching the gene scissors to detect RNA"},"content":{"rendered":"<p><a class=\"blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/a-new-addition-to-the-crispr-toolbox-teaching-the-gene-scissors-to-detect-rna.jpg\"><\/a><\/p>\n<p>CRISPR-Cas systems, defense systems in bacteria, have become a plentiful source of technologies for molecular diagnostics. Researchers at the Helmholtz Institute for RNA-based Infection Research (HIRI) in W\u00fcrzburg have expanded this extensive toolbox. Their novel method, called PUMA, enables the detection of RNA with Cas12 nucleases, which naturally target DNA. PUMA promises a wide range of applications and high accuracy.<\/p>\n<p>The team published its results in the journal Nature Communications (\u201cTracrRNA reprogramming enables direct PAM-independent detection of RNA with diverse DNA-targeting Cas12 nucleases\u201d).<\/p>\n<p>Bacteria have developed special defense mechanisms to protect themselves against viruses, which by no means infect only humans. As part of these so-called CRISPR-Cas systems, a CRISPR ribonucleic acid (crRNA), which serves as a \u201cguide RNA,\u201d recognizes regions of a foreign genome, such as viral DNA. The CRISPR-associated (Cas) nuclease, directed by a crRNA, then renders it harmless by cutting it like a pair of scissors.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CRISPR-Cas systems, defense systems in bacteria, have become a plentiful source of technologies for molecular diagnostics. Researchers at the Helmholtz Institute for RNA-based Infection Research (HIRI) in W\u00fcrzburg have expanded this extensive toolbox. Their novel method, called PUMA, enables the detection of RNA with Cas12 nucleases, which naturally target DNA. PUMA promises a wide range [\u2026]<\/p>\n","protected":false},"author":661,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,32],"tags":[],"class_list":["post-195216","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-education"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/195216","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/661"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=195216"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/195216\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=195216"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=195216"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=195216"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}