{"id":187552,"date":"2024-04-17T10:35:25","date_gmt":"2024-04-17T15:35:25","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2024\/04\/sparda-a-programmable-nucleic-acid-targeting-technology-like-crispr"},"modified":"2024-04-17T10:35:25","modified_gmt":"2024-04-17T15:35:25","slug":"sparda-a-programmable-nucleic-acid-targeting-technology-like-crispr","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2024\/04\/sparda-a-programmable-nucleic-acid-targeting-technology-like-crispr","title":{"rendered":"SPARDA: A Programmable Nucleic Acid Targeting Technology Like CRISPR"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/sparda-a-programmable-nucleic-acid-targeting-technology-like-crispr2.jpg\"><\/a><\/p>\n<p>An innovative programmable tool for targeting nucleic acids has been created, utilizing a prokaryotic immune defense system\u2014and it is not CRISPR-Cas. Russian Academy of Sciences researchers have successfully re-engineered prokaryotic Argonautes (pAgos) to utilize RNA guides for locating nucleic acid sequences. These systems have been modified to form a complex with effector nucleases.<\/p>\n<p>The researchers employed a two-component system known as SPARDA (short prokaryotic Argonaute, DNase, and RNase-associated) to effectively identify DNA sequences with a notable level of sensitivity and induce collateral nuclease activity. SPARDA and other concise pAgos systems that encode diverse effectors have the potential to offer a novel programmable tool for the field of biotechnology.<\/p>\n<p>The research article \u201c<a href=\"https:\/\/www.nature.com\/articles\/s41564-024-01654-5\">DNA-targeting short Argonautes complex with effector proteins for collateral nuclease activity and bacterial population immunity<\/a>\u201d was published in Nature Microbiology.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>An innovative programmable tool for targeting nucleic acids has been created, utilizing a prokaryotic immune defense system\u2014and it is not CRISPR-Cas. Russian Academy of Sciences researchers have successfully re-engineered prokaryotic Argonautes (pAgos) to utilize RNA guides for locating nucleic acid sequences. These systems have been modified to form a complex with effector nucleases. The researchers [\u2026]<\/p>\n","protected":false},"author":662,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,1522],"tags":[],"class_list":["post-187552","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-innovation"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/187552","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/662"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=187552"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/187552\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=187552"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=187552"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=187552"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}