{"id":187105,"date":"2024-04-10T14:24:01","date_gmt":"2024-04-10T19:24:01","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2024\/04\/masked-acid-chlorides-for-proximity-labelling-of-rna"},"modified":"2024-04-10T14:24:01","modified_gmt":"2024-04-10T19:24:01","slug":"masked-acid-chlorides-for-proximity-labelling-of-rna","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2024\/04\/masked-acid-chlorides-for-proximity-labelling-of-rna","title":{"rendered":"Masked acid chlorides for proximity labelling of RNA"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/masked-acid-chlorides-for-proximity-labelling-of-rna2.jpg\"><\/a><\/p>\n<p>A non-radical proximity labelling platform \u2014 BAP-seq \u2014 is presented that uses subcellular-localized BS2 esterase to convert unreactive enol-based probes into highly reactive acid chlorides in situ to label nearby RNAs. When paired with click-handle-mediated enrichment and sequencing, this chemistry enables high-resolution spatial mapping of RNAs across subcellular compartments.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A non-radical proximity labelling platform \u2014 BAP-seq \u2014 is presented that uses subcellular-localized BS2 esterase to convert unreactive enol-based probes into highly reactive acid chlorides in situ to label nearby RNAs. When paired with click-handle-mediated enrichment and sequencing, this chemistry enables high-resolution spatial mapping of RNAs across subcellular compartments.<\/p>\n","protected":false},"author":396,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[19,1965],"tags":[],"class_list":["post-187105","post","type-post","status-publish","format-standard","hentry","category-chemistry","category-mapping"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/187105","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/396"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=187105"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/187105\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=187105"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=187105"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=187105"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}