{"id":181076,"date":"2024-01-22T08:24:15","date_gmt":"2024-01-22T14:24:15","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2024\/01\/anti-crispr-proteins-can-regulate-cas3-targeted-deletions"},"modified":"2024-01-22T08:24:15","modified_gmt":"2024-01-22T14:24:15","slug":"anti-crispr-proteins-can-regulate-cas3-targeted-deletions","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2024\/01\/anti-crispr-proteins-can-regulate-cas3-targeted-deletions","title":{"rendered":"Anti-CRISPR Proteins Can Regulate Cas3 Targeted Deletions"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/anti-crispr-proteins-can-regulate-cas3-targeted-deletions.jpg\"><\/a><\/p>\n<p>The type I CRISPR protein Cas3 works like Pac-Man, chomping away at a continuous stream of nucleotides with intrinsic activity for introducing targeted large deletions from a few hundred base pairs to as large as 200 kb. However, without the molecular equivalent to the four colored ghosts who chase and capture Pac-Man, the broad and unidirectional genome editing activity of Cas3 is essentially unregulated.<\/p>\n<p>Yan Zhang, PhD, assistant professor in the department of biological chemistry at the University of Michigan Medical School, and her collaborators at Cornell University identified two anti-CRISPR proteins that can \u201cturn off\u201d Cas3, paving the way toward safer and better-controlled CRISPR applications.<\/p>\n<p>The research article, \u201c<a href=\"https:\/\/www.cell.com\/molecular-cell\/pdf\/S1097-2765(23)01080-8.pdf#%20\">Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications<\/a>,\u201d was published in Molecular Cell.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The type I CRISPR protein Cas3 works like Pac-Man, chomping away at a continuous stream of nucleotides with intrinsic activity for introducing targeted large deletions from a few hundred base pairs to as large as 200 kb. However, without the molecular equivalent to the four colored ghosts who chase and capture Pac-Man, the broad and [\u2026]<\/p>\n","protected":false},"author":661,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-181076","post","type-post","status-publish","format-standard","hentry","category-biotech-medical"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/181076","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/661"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=181076"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/181076\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=181076"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=181076"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=181076"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}