{"id":178315,"date":"2023-12-14T17:08:47","date_gmt":"2023-12-14T23:08:47","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2023\/12\/cas9-induced-targeted-integration-of-large-dna-payloads-in-primary-human-t-cells-via-homology-mediated-end-joining-dna-repair"},"modified":"2023-12-14T17:08:47","modified_gmt":"2023-12-14T23:08:47","slug":"cas9-induced-targeted-integration-of-large-dna-payloads-in-primary-human-t-cells-via-homology-mediated-end-joining-dna-repair","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2023\/12\/cas9-induced-targeted-integration-of-large-dna-payloads-in-primary-human-t-cells-via-homology-mediated-end-joining-dna-repair","title":{"rendered":"Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair"},"content":{"rendered":"<p style=\"padding-right: 20px\"><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/cas9-induced-targeted-integration-of-large-dna-payloads-in-primary-human-t-cells-via-homology-mediated-end-joining-dna-repair2.jpg\"><\/a><\/p>\n<p>The targeted integration of large DNA payloads into primary human T cells can be efficiently achieved non-virally by leveraging Cas9-based editing and the DNA-repair pathway homology-mediated end joining.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The targeted integration of large DNA payloads into primary human T cells can be efficiently achieved non-virally by leveraging Cas9-based editing and the DNA-repair pathway homology-mediated end joining.<\/p>\n","protected":false},"author":661,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-178315","post","type-post","status-publish","format-standard","hentry","category-biotech-medical"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/178315","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/661"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=178315"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/178315\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=178315"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=178315"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=178315"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}