{"id":177578,"date":"2023-12-07T04:24:15","date_gmt":"2023-12-07T10:24:15","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2023\/12\/phage-assisted-evolution-of-compact-cas9-variants-targeting-a-simple-nng-pam"},"modified":"2023-12-07T04:24:15","modified_gmt":"2023-12-07T10:24:15","slug":"phage-assisted-evolution-of-compact-cas9-variants-targeting-a-simple-nng-pam","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2023\/12\/phage-assisted-evolution-of-compact-cas9-variants-targeting-a-simple-nng-pam","title":{"rendered":"Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/phage-assisted-evolution-of-compact-cas9-variants-targeting-a-simple-nng-pam.jpg\"><\/a><\/p>\n<p>Qi <i>et al.<\/i> used phage-assisted evolution to optimize SlugCas9, a compact Cas9 nuclease, for NNG PAM recognition and developed a SlugCas9-NNG based adenine base editor for single AAV delivery.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Qi et al. used phage-assisted evolution to optimize SlugCas9, a compact Cas9 nuclease, for NNG PAM recognition and developed a SlugCas9-NNG based adenine base editor for single AAV delivery.<\/p>\n","protected":false},"author":661,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[385],"tags":[],"class_list":["post-177578","post","type-post","status-publish","format-standard","hentry","category-evolution"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/177578","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/661"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=177578"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/177578\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=177578"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=177578"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=177578"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}