{"id":172119,"date":"2023-09-15T21:25:12","date_gmt":"2023-09-16T02:25:12","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2023\/09\/targeted-evolution-of-adeno-associated-virus-capsids-for-systemic-transgene-delivery-to-microglia-and-tissue-resident-macrophages"},"modified":"2023-09-15T21:25:12","modified_gmt":"2023-09-16T02:25:12","slug":"targeted-evolution-of-adeno-associated-virus-capsids-for-systemic-transgene-delivery-to-microglia-and-tissue-resident-macrophages","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2023\/09\/targeted-evolution-of-adeno-associated-virus-capsids-for-systemic-transgene-delivery-to-microglia-and-tissue-resident-macrophages","title":{"rendered":"Targeted evolution of adeno-associated virus capsids for systemic transgene delivery to microglia and tissue-resident macrophages"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/targeted-evolution-of-adeno-associated-virus-capsids-for-systemic-transgene-delivery-to-microglia-and-tissue-resident-macrophages2.jpg\"><\/a><\/p>\n<p>Cool paper that adds a useful tool to the gene therapist\u2019s toolbox! Young et al. utilize an in vivo screening method to develop adeno-associated viruses (AAVs) which target microglia. They show that their AAVs transduce central nervous system microglia as well as tissue macrophages after intravenous injection. #biotechnology<\/p>\n<hr>\n<p>Tissue macrophages, including microglia, are notoriously resistant to genetic manipulation. Here, we report the creation of Adeno-associated viruses (AAV) variants that efficiently and widely transduce microglia and tissue macrophages in vivo following intravenous delivery, with transgene expression of up to 80%. We use this technology to demonstrate manipulation of microglia gene expression and microglial ablation, thereby providing invaluable research tools for the study of these important cells.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cool paper that adds a useful tool to the gene therapist\u2019s toolbox! Young et al. utilize an in vivo screening method to develop adeno-associated viruses (AAVs) which target microglia. They show that their AAVs transduce central nervous system microglia as well as tissue macrophages after intravenous injection. #biotechnology Tissue macrophages, including microglia, are notoriously resistant [\u2026]<\/p>\n","protected":false},"author":636,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,385,412],"tags":[],"class_list":["post-172119","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-evolution","category-genetics"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/172119","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/636"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=172119"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/172119\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=172119"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=172119"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=172119"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}