{"id":168944,"date":"2023-08-02T20:22:27","date_gmt":"2023-08-03T01:22:27","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2023\/08\/single-protein-nanopore-method-detects-post-translational-modifications"},"modified":"2023-08-02T20:22:27","modified_gmt":"2023-08-03T01:22:27","slug":"single-protein-nanopore-method-detects-post-translational-modifications","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2023\/08\/single-protein-nanopore-method-detects-post-translational-modifications","title":{"rendered":"Single-Protein Nanopore Method Detects Post-Translational Modifications"},"content":{"rendered":"

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At the University of Oxford, scientists have developed a nanopore technology that can identify three different post-translational modifications (PTMs) in individual proteins, even deep within long protein chains. The scientists asserted that their technology \u201c[lays] the groundwork for compiling inventories of the proteoforms in cells and tissues.\u201d<\/p>\n

The technology was introduced in Nature Nanotechnology<\/em>, in a paper titled, \u201cEnzyme-less nanopore detection of post-translational modifications within long polypeptides<\/a>.\u201d The paper noted that single-molecule proteoform identification requires knowledge of the architecture of long polypeptide chains, knowledge that has proven elusive. Although there are methods for translocating folded proteins through solid-state nanopores or protein nanopores of large sizes, these methods have yet to locate PTMs within a polypeptide sequence. The methods that have detected PTMs have been able to do so only within short peptides.<\/p>\n

In their paper, the Oxford scientists described their approach: We use electro-osmosis in an engineered charge-selective nanopore for the non-enzymatic capture, unfolding, and translocation of individual polypeptides of more than 1,200 residues. Unlabeled thioredoxin polyproteins undergo transport through the nanopore, with directional co-translocational unfolding occurring unit by unit from either the C or N terminus. Chaotropic reagents at non-denaturing concentrations accelerate the analysis.<\/p>\n","protected":false},"excerpt":{"rendered":"

At the University of Oxford, scientists have developed a nanopore technology that can identify three different post-translational modifications (PTMs) in individual proteins, even deep within long protein chains. The scientists asserted that their technology \u201c[lays] the groundwork for compiling inventories of the proteoforms in cells and tissues.\u201d The technology was introduced in Nature Nanotechnology, in [\u2026]<\/p>\n","protected":false},"author":662,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"class_list":["post-168944","post","type-post","status-publish","format-standard","hentry","category-futurism"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/168944","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/662"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=168944"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/168944\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=168944"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=168944"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=168944"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}