{"id":166135,"date":"2023-06-21T05:23:23","date_gmt":"2023-06-21T10:23:23","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2023\/06\/extreme-dna-resolution-spatially-multiplexed-single-molecule-translocations-through-a-nanopore-at-controlled-speeds"},"modified":"2023-06-21T05:23:23","modified_gmt":"2023-06-21T10:23:23","slug":"extreme-dna-resolution-spatially-multiplexed-single-molecule-translocations-through-a-nanopore-at-controlled-speeds","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2023\/06\/extreme-dna-resolution-spatially-multiplexed-single-molecule-translocations-through-a-nanopore-at-controlled-speeds","title":{"rendered":"Extreme DNA resolution: Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds"},"content":{"rendered":"<p><\/p>\n<p><iframe style=\"display: block; margin: 0 auto; width: 100%; aspect-ratio: 4\/3; object-fit: contain;\" src=\"https:\/\/www.youtube.com\/embed\/YeGZL0fKHtE?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope;\n   picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p>Aleksandra Radenovic, head of the Laboratory of Nanoscale Biology in the School of Engineering, has worked for years to improve nanopore technology, which involves passing a molecule like DNA through a tiny pore in a membrane to measure an ionic current. Scientists can determine DNA\u2019s sequence of nucleotides\u2014which encodes genetic information\u2014by analyzing how each one perturbs this current as it passes through. The research has been published in Nature Nanotechnology.<\/p>\n<p>Currently, the passage of molecules through a <a href=\"https:\/\/phys.org\/tags\/nanopore\/\" rel=\"tag\" class=\"\">nanopore<\/a> and the timing of their analysis are influenced by random physical forces, and the rapid movement of molecules makes achieving high analytical accuracy challenging. Radenovic has previously addressed these issues with optical tweezers and <a href=\"https:\/\/actu.epfl.ch\/news\/dna-sequencing-improved-by-slowing-down\/\">viscous liquids<\/a>. Now, a collaboration with Georg Fantner and his team in the Laboratory for Bio-and Nano-Instrumentation at EPFL has yielded the advancement she\u2019s been looking for\u2014with results that could go far beyond DNA.<\/p>\n<p>\u201cWe have combined the sensitivity of nanopores with the precision of scanning ion conductance microscopy (SICM), allowing us to lock onto specific molecules and locations and control how fast they move. This exquisite control could help fill a big gap in the field,\u201d Radenovic says. The researchers achieved this control using a repurposed state-of-the-art scanning ion conductance microscope, recently developed at the Lab for Bio-and Nano-Instrumentation.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Aleksandra Radenovic, head of the Laboratory of Nanoscale Biology in the School of Engineering, has worked for years to improve nanopore technology, which involves passing a molecule like DNA through a tiny pore in a membrane to measure an ionic current. Scientists can determine DNA\u2019s sequence of nucleotides\u2014which encodes genetic information\u2014by analyzing how each one [\u2026]<\/p>\n","protected":false},"author":367,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,412,4],"tags":[],"class_list":["post-166135","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-genetics","category-nanotechnology"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/166135","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/367"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=166135"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/166135\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=166135"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=166135"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=166135"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}