{"id":161224,"date":"2023-03-29T08:23:09","date_gmt":"2023-03-29T13:23:09","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2023\/03\/paste-dont-cut-genome-editing-tool-looks-beyond-crispr-and-prime"},"modified":"2023-03-29T08:23:09","modified_gmt":"2023-03-29T13:23:09","slug":"paste-dont-cut-genome-editing-tool-looks-beyond-crispr-and-prime","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2023\/03\/paste-dont-cut-genome-editing-tool-looks-beyond-crispr-and-prime","title":{"rendered":"PASTE, Don\u2019t Cut: Genome Editing Tool Looks Beyond CRISPR and Prime"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/paste-dont-cut-genome-editing-tool-looks-beyond-crispr-and-prime2.jpg\"><\/a><\/p>\n<p>A recently patented genome editing tool called PASTE holds genuine promise for expanding the universe of treatable genetic diseases. The approach combines elements of CRISPR and prime editing with a pair of enzymes designed to enable the integration of large segments of DNA without incurring double-stranded DNA breaks.<\/p>\n<p><a href=\"https:\/\/image-ppubs.uspto.gov\/dirsearch-public\/print\/downloadPdf\/11572556\">U.S. Patent No. 11,572,556<\/a>, assigned to MIT, covers systems, methods, and compositions for programmable addition via site-specific targeting elements (PASTE). The patent describes site-specific integration of a nucleic acid into a genome, using a CRISPR\u2013Cas9 nickase fused to a reverse transcriptase (RT) and a serine integrase. These enzymes target specific genome sequences known as attachment sites, binding to them before integrating their DNA payload.<\/p>\n<p>PASTE can insert DNA fragments as large as 50,000 base pairs, which puts it on a different plane compared to other genome editing tools such as prime editing.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A recently patented genome editing tool called PASTE holds genuine promise for expanding the universe of treatable genetic diseases. The approach combines elements of CRISPR and prime editing with a pair of enzymes designed to enable the integration of large segments of DNA without incurring double-stranded DNA breaks. U.S. Patent No. 11,572,556, assigned to MIT, [\u2026]<\/p>\n","protected":false},"author":661,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,412],"tags":[],"class_list":["post-161224","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-genetics"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/161224","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/661"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=161224"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/161224\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=161224"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=161224"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=161224"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}