{"id":160914,"date":"2023-03-24T10:23:15","date_gmt":"2023-03-24T15:23:15","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2023\/03\/researchers-develop-method-for-deciphering-positional-rules-in-splicing"},"modified":"2023-03-24T10:23:15","modified_gmt":"2023-03-24T15:23:15","slug":"researchers-develop-method-for-deciphering-positional-rules-in-splicing","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2023\/03\/researchers-develop-method-for-deciphering-positional-rules-in-splicing","title":{"rendered":"Researchers develop method for deciphering positional rules in splicing"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/researchers-develop-method-for-deciphering-positional-rules-in-splicing2.jpg\"><\/a><\/p>\n<p>A research team led by Prof. Xue Yuanchao from the Institute of Biophysics of the Chinese Academy of Sciences has developed a new method for global profiling of in-situ RNA\u2013RNA contacts associated with a specific RNA-binding protein (RBP) and revealed positional mechanisms by which PTBP1-associated RNA loops regulate cassette exon splicing.<\/p>\n<p>This study was published online in <i>Molecular Cell<\/i> on March 22.<\/p>\n<p>In <a href=\"https:\/\/phys.org\/tags\/eukaryotes\/\" rel=\"tag\" class=\"\">eukaryotes<\/a>, the same pre-mRNA can produce multiple protein isoforms to execute similar or different biological functions through <a href=\"https:\/\/phys.org\/tags\/alternative+splicing\/\" rel=\"tag\" class=\"\">alternative splicing<\/a>. Several longstanding models proposed that RBPs may regulate alternative splicing by modulating long-range RNA\u2013RNA interactions (RRI). However, direct experimental evidence was lacking.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A research team led by Prof. Xue Yuanchao from the Institute of Biophysics of the Chinese Academy of Sciences has developed a new method for global profiling of in-situ RNA\u2013RNA contacts associated with a specific RNA-binding protein (RBP) and revealed positional mechanisms by which PTBP1-associated RNA loops regulate cassette exon splicing. This study was published [\u2026]<\/p>\n","protected":false},"author":661,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-160914","post","type-post","status-publish","format-standard","hentry","category-biological"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/160914","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/661"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=160914"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/160914\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=160914"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=160914"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=160914"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}