{"id":151962,"date":"2022-12-06T01:23:29","date_gmt":"2022-12-06T07:23:29","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2022\/12\/team-develops-photon-efficient-volumetric-imaging-method-with-light-sheet-scanning-fluorescence-microscopy"},"modified":"2022-12-06T01:23:29","modified_gmt":"2022-12-06T07:23:29","slug":"team-develops-photon-efficient-volumetric-imaging-method-with-light-sheet-scanning-fluorescence-microscopy","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2022\/12\/team-develops-photon-efficient-volumetric-imaging-method-with-light-sheet-scanning-fluorescence-microscopy","title":{"rendered":"Team develops photon-efficient volumetric imaging method with light-sheet scanning fluorescence microscopy"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/team-develops-photon-efficient-volumetric-imaging-method-with-light-sheet-scanning-fluorescence-microscopy2.jpg\"><\/a><\/p>\n<p>In biological imaging, researchers aim to achieve 3D, high-speed, and high-resolution, with low photobleaching and phototoxicity. The light-sheet fluorescence microscope (LSFM) helps meet that aim. Based on a unique excitation and detection scheme, the LSFM can image live specimens with high spatiotemporal resolution and low photobleaching. It has shown great potential for 3D imaging of biological samples.<\/p>\n<p>The principle of LSFM technology is to illuminate the sample with a thin <a href=\"https:\/\/phys.org\/tags\/light-sheet\/\" rel=\"tag\" class=\"\">light-sheet<\/a> and then collect the emitted fluorescence along the axis perpendicular to the transmission of the light-sheet. Therefore, only fluorophores close to the <a href=\"https:\/\/phys.org\/tags\/focal+plane\/\" rel=\"tag\" class=\"\">focal plane<\/a> are excited and detected. Using a thinner light-sheet improves the axial <a href=\"https:\/\/phys.org\/tags\/resolution\/\" rel=\"tag\" class=\"\">resolution<\/a>, while a longer light-sheet improves the <a href=\"https:\/\/phys.org\/tags\/field+of+view\/\" rel=\"tag\" class=\"\">field of view<\/a> (FoV) and imaging speed. Tradeoffs are required, as it is difficult to generate a thin, uniform light-sheet.<\/p>\n<p>Multiple light-sheets can be tiled to generate a virtual light-sheet with a higher aspect ratio. However, multiple beams also introduce sidelobes, decreasing the axial resolution and optical sectioning. Axially swept light-sheet microscopy (ASLM) uses a slit to reject the sidelobes. It uses the rolling shutter of the sCMOS, which naturally serves as a slit, to synchronize beam scanning. ASLM can image an arbitrarily large FoV with optimal axial resolution. However, the fluorescence signal outside the rolling shutter will be rejected, so a larger FoV comes at the price of lower photon efficiency.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In biological imaging, researchers aim to achieve 3D, high-speed, and high-resolution, with low photobleaching and phototoxicity. The light-sheet fluorescence microscope (LSFM) helps meet that aim. Based on a unique excitation and detection scheme, the LSFM can image live specimens with high spatiotemporal resolution and low photobleaching. It has shown great potential for 3D imaging of [\u2026]<\/p>\n","protected":false},"author":427,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-151962","post","type-post","status-publish","format-standard","hentry","category-biological"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/151962","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/427"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=151962"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/151962\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=151962"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=151962"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=151962"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}