{"id":148208,"date":"2022-10-15T01:25:28","date_gmt":"2022-10-15T06:25:28","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2022\/10\/electroporation-and-cell-killing"},"modified":"2022-10-15T01:25:28","modified_gmt":"2022-10-15T06:25:28","slug":"electroporation-and-cell-killing","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2022\/10\/electroporation-and-cell-killing","title":{"rendered":"Electroporation and cell killing"},"content":{"rendered":"

<\/a><\/p>\n

All strength-duration curves expectedly climbed as pulse duration decreased (Fig. 9 <\/a>A). The nerve stimulation thresholds rose much faster (similar to findings with H-FIRE17<\/a><\/sup>), thereby decreasing the ratio of cell killing and excitation thresholds for shorter pulses (Fig. 9 <\/a>B). Nanosecond pulses showed a profound and unequivocal advantage over conventional 100-\u00b5s pulses: The ratio of cell killing and excitation thresholds dropped from as high as 500 for unipolar 100-\u00b5s pulses to 13 for unipolar 150-ns pulses. Engaging bipolar cancellation further decreased this ratio to only 4 for bipolar 150 ns pulses (Fig. 9 <\/a>B). Thus, bipolar nanosecond pulses are the best choice for the reduction of neuromuscular stimulation from PEF ablation.<\/p>\n

The stimulation boundaries for ablation with different pulse durations and shapes are illustrated by a hypothetical example in Fig. 10<\/a>. Based on data in Fig. 9<\/a> and numerical simulations of the electric field distribution, we explored how far electrostimulation will reach from an ablation area. A tumor 0.7 cm in diameter was placed between two needle electrodes (0.5-cm diameter, 1.7-cm center-to-center distance) for ablation by PEF, and 300 pulses were delivered at the minimum amplitude needed for tumor ablation. Nerve stimulation occurred within a range of about 2 cm for bipolar 200-ns pulses, but as far as 20 cm away for unipolar 100-\u00b5s pulses.<\/p>\n","protected":false},"excerpt":{"rendered":"

All strength-duration curves expectedly climbed as pulse duration decreased (Fig. 9 A). The nerve stimulation thresholds rose much faster (similar to findings with H-FIRE17), thereby decreasing the ratio of cell killing and excitation thresholds for shorter pulses (Fig. 9 B). Nanosecond pulses showed a profound and unequivocal advantage over conventional 100-\u00b5s pulses: The ratio of [\u2026]<\/p>\n","protected":false},"author":513,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"class_list":["post-148208","post","type-post","status-publish","format-standard","hentry","category-futurism"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/148208","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/513"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=148208"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/148208\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=148208"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=148208"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=148208"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}