{"id":146709,"date":"2022-09-20T11:02:39","date_gmt":"2022-09-20T16:02:39","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2022\/09\/engineered-cells-become-drug-factories-with-avian-assistance"},"modified":"2022-09-20T11:02:39","modified_gmt":"2022-09-20T16:02:39","slug":"engineered-cells-become-drug-factories-with-avian-assistance","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2022\/09\/engineered-cells-become-drug-factories-with-avian-assistance","title":{"rendered":"Engineered Cells Become Drug Factories with Avian Assistance"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/engineered-cells-become-drug-factories-with-avian-assistance2.jpg\"><\/a><\/p>\n<p>The genetic encoding of ncAAs with distinct chemical, biological, and physical properties requires the engineering of bioorthogonal translational machinery, consisting of an evolved aminoacyl-tRNA synthetase\/tRNA pair and a \u201cblank\u201d codon. To achieve this, the researchers mimicked the ibis\u2019 ability to synthesize sTyr and incorporate it into proteins.<\/p>\n<p>The Xiao lab employed a mutant amber stop codon to encode the desired sulfotransferase, resulting in a completely autonomous mammalian cell line capable of biosynthesizing sTyr and incorporating it with great precision into proteins.<\/p>\n<p>These engineered cells, the authors wrote, can produce \u201csite-specifically sulfated proteins at a higher yield than cells fed exogenously with the highest level of sTyr reported in the literature.\u201d They used the cells to prepare highly potent thrombin inhibitors with site-specific sulfation.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The genetic encoding of ncAAs with distinct chemical, biological, and physical properties requires the engineering of bioorthogonal translational machinery, consisting of an evolved aminoacyl-tRNA synthetase\/tRNA pair and a \u201cblank\u201d codon. To achieve this, the researchers mimicked the ibis\u2019 ability to synthesize sTyr and incorporate it into proteins. The Xiao lab employed a mutant amber stop [\u2026]<\/p>\n","protected":false},"author":599,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1902,11,19,412,6],"tags":[],"class_list":["post-146709","post","type-post","status-publish","format-standard","hentry","category-bioengineering","category-biotech-medical","category-chemistry","category-genetics","category-robotics-ai"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/146709","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/599"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=146709"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/146709\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=146709"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=146709"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=146709"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}