{"id":144206,"date":"2022-08-16T00:53:09","date_gmt":"2022-08-16T05:53:09","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2022\/08\/immortal-human-pancreatic-duct-epithelial-cell-lines-with-near-normal-genotype-and-phenotype"},"modified":"2022-08-16T00:53:09","modified_gmt":"2022-08-16T05:53:09","slug":"immortal-human-pancreatic-duct-epithelial-cell-lines-with-near-normal-genotype-and-phenotype","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2022\/08\/immortal-human-pancreatic-duct-epithelial-cell-lines-with-near-normal-genotype-and-phenotype","title":{"rendered":"Immortal Human Pancreatic Duct Epithelial Cell Lines with Near Normal Genotype and Phenotype"},"content":{"rendered":"<p style=\"padding-right: 20px\"><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/immortal-human-pancreatic-duct-epithelial-cell-lines-with-near-normal-genotype-and-phenotype2.jpg\"><\/a><\/p>\n<p>Circa 2000<\/p>\n<hr>\n<p>Immortal epithelial cell lines were previously established after transduction of the HPV16-E6E7 genes into primary cultures of normal pancreatic duct epithelial cells. Single clones were isolated that demonstrated near normal genotype and phenotype. The proliferation of HPDE6-E6E7c7 and c11 cells is anchorage-dependent, and they were nontumorigenic in SCID mice. The cell lines demonstrated many phenotypes of normal pancreatic duct epithelium, including mRNA expression of carbonic anhydrase II, MUC-1, and cytokeratins 7, 8, 18, and 19.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Circa 2000 Immortal epithelial cell lines were previously established after transduction of the HPV16-E6E7 genes into primary cultures of normal pancreatic duct epithelial cells. Single clones were isolated that demonstrated near normal genotype and phenotype. The proliferation of HPDE6-E6E7c7 and c11 cells is anchorage-dependent, and they were nontumorigenic in SCID mice. The cell lines demonstrated [\u2026]<\/p>\n","protected":false},"author":513,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[269],"tags":[],"class_list":["post-144206","post","type-post","status-publish","format-standard","hentry","category-life-extension"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/144206","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/513"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=144206"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/144206\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=144206"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=144206"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=144206"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}