{"id":142368,"date":"2022-07-17T02:26:17","date_gmt":"2022-07-17T07:26:17","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2022\/07\/the-lightcycler-480-real-time-pcr-system-a-versatile-platform-for-genetic-variation-research"},"modified":"2022-07-17T02:26:17","modified_gmt":"2022-07-17T07:26:17","slug":"the-lightcycler-480-real-time-pcr-system-a-versatile-platform-for-genetic-variation-research","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2022\/07\/the-lightcycler-480-real-time-pcr-system-a-versatile-platform-for-genetic-variation-research","title":{"rendered":"The LightCycler\u00ae 480 real-time PCR system: a versatile platform for genetic variation research"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/the-lightcycler-480-real-time-pcr-system-a-versatile-platform-for-genetic-variation-research2.jpg\"><\/a><\/p>\n<p>Circa 2008<\/p>\n<hr>\n<p>From endpoint fluorescence to melting curve analysis.<\/p>\n<p>Today\u2019s research on somatic, genetic and epigenetic variation in eukaryotic cells requires fast, accurate and cost-effective methods for screening large numbers of samples or loci in parallel. Variations identified by genomic sequencing or array studies need to be subsequently confirmed and validated.<\/p>\n<p>Real-time PCR has become a well established technology for this purpose. The plate-based LightCycler 480 system offers a broad selection of methods and applications (<a data-track=\"click\" data-track-label=\"link\" data-track-action=\"figure anchor\" href=\"https:\/\/www.nature.com\/articles\/nmeth.f.207#Fig1\">Fig. 1<\/a>).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Circa 2008 From endpoint fluorescence to melting curve analysis. Today\u2019s research on somatic, genetic and epigenetic variation in eukaryotic cells requires fast, accurate and cost-effective methods for screening large numbers of samples or loci in parallel. Variations identified by genomic sequencing or array studies need to be subsequently confirmed and validated. Real-time PCR has become [\u2026]<\/p>\n","protected":false},"author":513,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[412],"tags":[],"class_list":["post-142368","post","type-post","status-publish","format-standard","hentry","category-genetics"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/142368","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/513"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=142368"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/142368\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=142368"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=142368"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=142368"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}