{"id":128464,"date":"2021-10-02T20:23:07","date_gmt":"2021-10-03T03:23:07","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2021\/10\/digenome-seq-genome-wide-profiling-of-crispr-cas9-off-target-effects-in-human-cells"},"modified":"2021-10-02T20:23:07","modified_gmt":"2021-10-03T03:23:07","slug":"digenome-seq-genome-wide-profiling-of-crispr-cas9-off-target-effects-in-human-cells","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2021\/10\/digenome-seq-genome-wide-profiling-of-crispr-cas9-off-target-effects-in-human-cells","title":{"rendered":"Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells"},"content":{"rendered":"<p style=\"padding-right: 20px\"><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/digenome-seq-genome-wide-profiling-of-crispr-cas9-off-target-effects-in-human-cells.jpg\"><\/a><\/p>\n<p><i>In vitro<\/i> digestion of genomic DNA with Cas9 and single guide RNAs (sgRNAs) yields genome-wide off-target sites at frequencies below 0.1%. Off-target sites can be further reduced with modified sgRNAs.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In vitro digestion of genomic DNA with Cas9 and single guide RNAs (sgRNAs) yields genome-wide off-target sites at frequencies below 0.1%. Off-target sites can be further reduced with modified sgRNAs.<\/p>\n","protected":false},"author":621,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-128464","post","type-post","status-publish","format-standard","hentry","category-biotech-medical"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/128464","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/621"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=128464"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/128464\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=128464"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=128464"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=128464"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}