{"id":123589,"date":"2021-06-08T21:23:30","date_gmt":"2021-06-09T04:23:30","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2021\/06\/aldehyde-stabilized-cryopreservation"},"modified":"2021-06-08T21:23:30","modified_gmt":"2021-06-09T04:23:30","slug":"aldehyde-stabilized-cryopreservation","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2021\/06\/aldehyde-stabilized-cryopreservation","title":{"rendered":"Aldehyde-stabilized cryopreservation"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/aldehyde-stabilized-cryopreservation.jpg\"><\/a><\/p>\n<p>Circa 2015 brain immortality through aldehyde stabilized cryopreservation.<\/p>\n<hr>\n<p>We describe here a new cryobiological and neurobiological technique, aldehyde-stabilized <a href=\"https:\/\/www.sciencedirect.com\/topics\/biochemistry-genetics-and-molecular-biology\/cryopreservation\" title=\"Learn more about Cryopreservation from ScienceDirect's AI-generated Topic Pages\" class=\"\">cryopreservation<\/a> (ASC), which demonstrates the relevance and utility of advanced cryopreservation science for the neurobiological research community. ASC is a new brain-banking technique designed to facilitate neuroanatomic research such as <a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/connectome\" title=\"Learn more about Connectome from ScienceDirect's AI-generated Topic Pages\" class=\"\">connectomics<\/a> research, and has the unique ability to combine stable long term ice-free sample storage with excellent anatomical resolution. To demonstrate the feasibility of ASC, we perfuse-fixed rabbit and pig brains with a glutaraldehyde-based fixative, then slowly perfused increasing concentrations of <a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/ethylene-glycol\" title=\"Learn more about Ethylene Glycol from ScienceDirect's AI-generated Topic Pages\" class=\"\">ethylene glycol<\/a> over several hours in a manner similar to techniques used for whole organ cryopreservation. Once 65% w\/v ethylene glycol was reached, we vitrified brains at \u2212135 \u00b0C for indefinite long-term storage. Vitrified brains were rewarmed and the <a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/cryoprotectant\" title=\"Learn more about Cryoprotectant from ScienceDirect's AI-generated Topic Pages\" class=\"\">cryoprotectant<\/a> removed either by perfusion or gradual diffusion from brain slices. We evaluated ASC-processed brains by <a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/electron-microscopy\" title=\"Learn more about Electron Microscopy from ScienceDirect's AI-generated Topic Pages\" class=\"\">electron microscopy<\/a> of multiple regions across the whole brain and by <a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/focused-ion-beam\" title=\"Learn more about Focused Ion Beam from ScienceDirect's AI-generated Topic Pages\" class=\"\">Focused Ion Beam<\/a> Milling and Scanning Electron Microscopy (FIB-SEM) imaging of selected brain volumes. Preservation was uniformly excellent: processes were easily traceable and synapses were crisp in both species. Aldehyde-stabilized cryopreservation has many advantages over other brain-banking techniques: chemicals are delivered via perfusion, which enables easy scaling to brains of any size; vitrification ensures that the <a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/ultrastructure\" title=\"Learn more about Ultrastructure from ScienceDirect's AI-generated Topic Pages\" class=\"\">ultrastructure<\/a> of the brain will not degrade even over very long storage times; and the cryoprotectant can be removed, yielding a perfusable aldehyde-preserved brain which is suitable for a wide variety of brain assays.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Circa 2015 brain immortality through aldehyde stabilized cryopreservation. We describe here a new cryobiological and neurobiological technique, aldehyde-stabilized cryopreservation (ASC), which demonstrates the relevance and utility of advanced cryopreservation science for the neurobiological research community. ASC is a new brain-banking technique designed to facilitate neuroanatomic research such as connectomics research, and has the unique ability [\u2026]<\/p>\n","protected":false},"author":513,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,19,2017,45,269,47],"tags":[],"class_list":["post-123589","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-chemistry","category-cryonics","category-finance","category-life-extension","category-neuroscience"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/123589","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/513"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=123589"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/123589\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=123589"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=123589"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=123589"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}