{"id":115032,"date":"2020-10-24T20:27:28","date_gmt":"2020-10-25T03:27:28","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2020\/10\/new-crispr-based-imaging-tool-is-going-to-be-hiuge"},"modified":"2020-10-24T20:27:28","modified_gmt":"2020-10-25T03:27:28","slug":"new-crispr-based-imaging-tool-is-going-to-be-hiuge","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2020\/10\/new-crispr-based-imaging-tool-is-going-to-be-hiuge","title":{"rendered":"New CRISPR-Based Imaging Tool Is Going to Be HiUGE"},"content":{"rendered":"<p><\/p>\n<p><iframe style=\"display: block; margin: 0 auto; width: 100%; aspect-ratio: 4\/3; object-fit: contain;\" src=\"https:\/\/www.youtube.com\/embed\/I4EdWb37-Y0?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope;\n   picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p>A team of researchers at Duke University have developed an imaging technology for tagging structures at a cellular level that overcomes the shortcomings of existing antibody-based techniques. Immunofluorescence imaging is a key part of the cell biologist\u2019s toolbox, in which a fluorescent \u2018flare\u2019 attached to an antibody allows them to visualize the presence of specific target proteins in cell or tissue samples. The issue is that this specificity isn\u2019t always 100 percent \u2014 sometimes the antibodies bind to other closely related proteins as well, making it difficult to interpret the results.<\/p>\n<p>Duke\u2019s cell biology chair <a href=\"https:\/\/scholars.duke.edu\/person\/s.soderling\">Scott Soderling<\/a> has led a team that developed Homology-independent Universal Genome Engineering (HiUGE), an innovation that uses gene-editing technology to rise above the shortcomings of traditional commercial antibodies for imaging.<\/p>\n<p>\u201cWe had this idea that CRISPR could be a really amazing tool to address the pressing problem of trying to identify and label these hundreds of proteins,\u201d said Soderling.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A team of researchers at Duke University have developed an imaging technology for tagging structures at a cellular level that overcomes the shortcomings of existing antibody-based techniques. Immunofluorescence imaging is a key part of the cell biologist\u2019s toolbox, in which a fluorescent \u2018flare\u2019 attached to an antibody allows them to visualize the presence of specific [\u2026]<\/p>\n","protected":false},"author":609,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1902,11,412],"tags":[],"class_list":["post-115032","post","type-post","status-publish","format-standard","hentry","category-bioengineering","category-biotech-medical","category-genetics"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/115032","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/609"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=115032"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/115032\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=115032"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=115032"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=115032"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}