{"id":102338,"date":"2020-02-13T17:12:23","date_gmt":"2020-02-14T01:12:23","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2020\/02\/the-pan-genome-effector-triggered-immunity-landscape-of-a-host-pathogen-interaction"},"modified":"2020-02-13T17:12:23","modified_gmt":"2020-02-14T01:12:23","slug":"the-pan-genome-effector-triggered-immunity-landscape-of-a-host-pathogen-interaction","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2020\/02\/the-pan-genome-effector-triggered-immunity-landscape-of-a-host-pathogen-interaction","title":{"rendered":"The pan-genome effector-triggered immunity landscape of a host-pathogen interaction"},"content":{"rendered":"<p style=\"padding-right: 20px\"><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/the-pan-genome-effector-triggered-immunity-landscape-of-a-host-pathogen-interaction.jpg\"><\/a><\/p>\n<p>Plant pathogens elicit an immune response through effector proteins. In turn, plant genomes encode genes that determine species-specific recognition of these effectors by a process known collectively as effector-triggered immunity (ETI). By examining a range of strains of the pathogen <em><i>Pseudomonas syringae<\/i><\/em> that infect the model plant <em><i>Arabidopsis thaliana<\/i><\/em>, Laflamme <em><i>et al.<\/i><\/em> generated a <em><i>P. syringae<\/i><\/em> Type III Effector Compendium (PsyTEC) and in turn identified the genes responsible for ETI in <em><i>Arabidopsis.<\/i><\/em> This pan-genome analysis revealed that relatively few <em><i>A. thaliana<\/i><\/em> genes are responsible for recognizing the majority of <em><i>P. syringae<\/i><\/em> effectors. These results provide insight into why most pathogenic microbes only infect specific plant species.<\/p>\n<p><em><i>Science<\/i><\/em>, this issue p. <a href=\"https:\/\/science.sciencemag.org\/lookup\/doi\/10.1126\/science.aax4079\">763<\/a><\/p>\n<p>Effector-triggered immunity (ETI), induced by host immune receptors in response to microbial effectors, protects plants against virulent pathogens. However, a systematic study of ETI prevalence against species-wide pathogen diversity is lacking. We constructed the <em><i>Pseudomonas syringae<\/i><\/em> Type III Effector Compendium (PsyTEC) to reduce the pan-genome complexity of 5127 unique effector proteins, distributed among 70 families from 494 strains, to 529 representative alleles. We screened PsyTEC on the model plant <em><i>Arabidopsis thaliana<\/i><\/em> and identified 59 ETI-eliciting alleles (11.2%) from 19 families (27.1%), with orthologs distributed among 96.8% of <em><i>P. syringae<\/i><\/em> strains. We also identified two previously undescribed host immune receptors, including CAR1, which recognizes the conserved effectors AvrE and HopAA1, and found that 94.7% of strains harbor alleles predicted to be recognized by either CAR1 or ZAR1.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Plant pathogens elicit an immune response through effector proteins. In turn, plant genomes encode genes that determine species-specific recognition of these effectors by a process known collectively as effector-triggered immunity (ETI). By examining a range of strains of the pathogen Pseudomonas syringae that infect the model plant Arabidopsis thaliana, Laflamme et al. generated a P. [\u2026]<\/p>\n","protected":false},"author":396,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-102338","post","type-post","status-publish","format-standard","hentry","category-biotech-medical"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/102338","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/396"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=102338"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/102338\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=102338"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=102338"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=102338"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}