{"id":101853,"date":"2020-02-04T00:23:14","date_gmt":"2020-02-04T08:23:14","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2020\/02\/keth-seq-for-transcriptome-wide-rna-structure-mapping"},"modified":"2020-02-04T00:23:14","modified_gmt":"2020-02-04T08:23:14","slug":"keth-seq-for-transcriptome-wide-rna-structure-mapping","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2020\/02\/keth-seq-for-transcriptome-wide-rna-structure-mapping","title":{"rendered":"Keth-seq for transcriptome-wide RNA structure mapping"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/keth-seq-for-transcriptome-wide-rna-structure-mapping.jpg\"><\/a><\/p>\n<p>RNA secondary structure is critical to RNA regulation and function. We report a new N3-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N3-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping. The authors designed a chemical probe, azido-kethoxal, to specifically label guanosine in single-strand RNAs in live cells that could be used to determine transcriptome-wide RNA secondary structures.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>RNA secondary structure is critical to RNA regulation and function. We report a new N3-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N3-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping. The authors designed a chemical probe, azido-kethoxal, to specifically label [\u2026]<\/p>\n","protected":false},"author":396,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[19,1965],"tags":[],"class_list":["post-101853","post","type-post","status-publish","format-standard","hentry","category-chemistry","category-mapping"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/101853","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/396"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=101853"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/101853\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=101853"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=101853"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=101853"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}