{"id":100987,"date":"2020-01-17T08:49:43","date_gmt":"2020-01-17T16:49:43","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2020\/01\/microscopy-technique-reveals-cells-3d-ultrastructure-in-new-detail"},"modified":"2020-01-17T08:49:43","modified_gmt":"2020-01-17T16:49:43","slug":"microscopy-technique-reveals-cells-3d-ultrastructure-in-new-detail","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2020\/01\/microscopy-technique-reveals-cells-3d-ultrastructure-in-new-detail","title":{"rendered":"Microscopy technique reveals cells\u2019 3D ultrastructure in new detail"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/microscopy-technique-reveals-cells-3d-ultrastructure-in-new-detail.jpg\"><\/a><\/p>\n<p>Inside a cell, tentacled vesicles shuttle cargo for sorting. DNA rearranges in the nucleus as stem cells differentiate into neurons. Neighboring neurons cling to one another through a web-like interface. And a new microscopy technique shows it all, in exquisite detail.<\/p>\n<p>The technique, called cryo-SR\/EM, melds images captured from <a href=\"https:\/\/phys.org\/tags\/electron+microscopes\/\" rel=\"tag\" class=\"\">electron microscopes<\/a> and super-resolution light microscopes, resulting in brilliant, clear detailed views of the inside of <a href=\"https:\/\/phys.org\/tags\/cells\/\" rel=\"tag\" class=\"\">cells<\/a>\u2014in 3D.<\/p>\n<p>For years, scientists have probed the microscopic world inside cells, developing new tools to view these basic units of life. But each tool comes with a tradeoff. Light microscopy makes it simple to identify specific cellular structures by tagging them with easy-to-see fluorescent molecules. With the development of super-resolution (SR) <a href=\"https:\/\/phys.org\/tags\/fluorescence+microscopy\/\" rel=\"tag\" class=\"\">fluorescence microscopy<\/a>, these structures can be viewed with even greater clarity. But fluorescence can reveal only a few of the more than 10,000 proteins in a cell at a given time, making it difficult to understand how these few relate to everything else. Electron microscopy (EM), on the other hand, reveals all cellular structures in high-resolution pictures\u2014but delineating one feature from all others by EM alone can be difficult because the space inside of cells is so crowded.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Inside a cell, tentacled vesicles shuttle cargo for sorting. DNA rearranges in the nucleus as stem cells differentiate into neurons. Neighboring neurons cling to one another through a web-like interface. And a new microscopy technique shows it all, in exquisite detail. The technique, called cryo-SR\/EM, melds images captured from electron microscopes and super-resolution light microscopes, [\u2026]<\/p>\n","protected":false},"author":535,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11,47],"tags":[],"class_list":["post-100987","post","type-post","status-publish","format-standard","hentry","category-biotech-medical","category-neuroscience"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/100987","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/535"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=100987"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/100987\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=100987"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=100987"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=100987"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}