{"id":100913,"date":"2020-01-15T22:47:35","date_gmt":"2020-01-16T06:47:35","guid":{"rendered":"https:\/\/lifeboat.com\/blog\/2020\/01\/figure-1-a-crispr-cas9-genetically-engineered-mouse-model-for-mers-cov-replication"},"modified":"2020-01-15T22:47:35","modified_gmt":"2020-01-16T06:47:35","slug":"figure-1-a-crispr-cas9-genetically-engineered-mouse-model-for-mers-cov-replication","status":"publish","type":"post","link":"https:\/\/lifeboat.com\/blog\/2020\/01\/figure-1-a-crispr-cas9-genetically-engineered-mouse-model-for-mers-cov-replication","title":{"rendered":"Figure 1: A CRISPR\u2013Cas9 genetically engineered mouse model for MERS-CoV replication"},"content":{"rendered":"<p><a class=\"aligncenter blog-photo\" href=\"https:\/\/lifeboat.com\/blog.images\/figure-1-a-crispr-cas9-genetically-engineered-mouse-model-for-mers-cov-replication2.jpg\"><\/a><\/p>\n<p>A, C57BL\/6J mice were genetically engineered using CRISPR\u2013Cas9 genomic editing to encode 288L and 330R in mDPP4 on one chromosome (heterozygous, 288\/330<sup>+\/\u2212<\/sup>) or on both chromosomes (homozygous, 288\/330<sup>+\/+<\/sup>). b, Northern blot of mDPP4 mRNA expression. c, Immunohistochemistry (IHC) of mDPP4 protein in the lungs, brain and kidneys of individual C57BL\/6J wild-type (WT), 288\/330<sup>+\/\u2212<\/sup> and 288\/330<sup>+\/+<\/sup> mice. d, Viral titres for MERS-CoV at 3 days post-infection from C57BL\/6J WT, 288\/330<sup>+\/\u2212<\/sup> and 288\/330<sup>+\/+<\/sup> (all n = 4) mice infected with 5 \u00d7 10<sup>5<\/sup> plaque-forming units (p.f.u.) of the indicated viruses. Bar graphs show means + s.d.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A, C57BL\/6J mice were genetically engineered using CRISPR\u2013Cas9 genomic editing to encode 288L and 330R in mDPP4 on one chromosome (heterozygous, 288\/330+\/\u2212) or on both chromosomes (homozygous, 288\/330+\/+). b, Northern blot of mDPP4 mRNA expression. c, Immunohistochemistry (IHC) of mDPP4 protein in the lungs, brain and kidneys of individual C57BL\/6J wild-type (WT), 288\/330+\/\u2212 and 288\/330+\/+ [\u2026]<\/p>\n","protected":false},"author":513,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1902,11,19,412,47],"tags":[],"class_list":["post-100913","post","type-post","status-publish","format-standard","hentry","category-bioengineering","category-biotech-medical","category-chemistry","category-genetics","category-neuroscience"],"_links":{"self":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/100913","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/users\/513"}],"replies":[{"embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/comments?post=100913"}],"version-history":[{"count":0,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/posts\/100913\/revisions"}],"wp:attachment":[{"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/media?parent=100913"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/categories?post=100913"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lifeboat.com\/blog\/wp-json\/wp\/v2\/tags?post=100913"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}