February’s Longevity Review is now available, with a look at how the combination of Omega 3, Vit D & exercise can slow aging; a human trial of young blood plasma; Klotho gene therapy to increase lifespan; and, in the Canadian content study, how cellular reprogramming protects retinal neurons in multiple sclerosis.

A review of the most interesting and impactful longevity related studies from Febuary, with a look at how the combination of Omega 3, Vit D & exercise can slow aging; a human trial of young blood plasma; Klotho gene therapy to increase lifespan; and, in the Canadian content study, how cellular reprogramming protects retinal neurons in multiple sclerosis.

Contents:

1. Intro 0:00
2. Omega 3, Vitamin D & Exercise Slow Aging 0:55
3. Young Blood Plasma Human Trial 9:46
4. Klotho Gene Therapy Increases Lifespan 18:45.

Canadian Content Study.

“.

Gene therapy was used to increase levels of Klotho, one of the first longevity genes discovered in the 1990’s, in mice. This led to improved physical fitness, muscular regenerative capacity, bone micrstructure, neurogenesis and a 20% increase in lifespan.

Contents:

Intro 0:00
Figure 1 — Longevity experiment follow-up and AAV treatment effectivity assessment 1:20
Figure 2 — Physical tests and histological analysis of muscular tissue from treated naturally aged animals 4:04
Figure 3 — Histological analysis of muscle from s-KL-treated animals 7 days after transplantation 6:27
Figure 4 — MicroCT structural and gene expression analysis in treated bones of 24-MO mice 7:34
Figure 5 — Histological analysis of Iba1, GFAP, and neurogenesis markers in CNS from aged animals 8:52
Figure 6 — Transcriptomic analysis of hippocampal RNA-seq data 9:59
Graphical Abstract & Conclusion 11:04.

Study reviewed:

Longevity snapshot 7 — cellular rejuvenation protects neurons.

Reviewing a Canadian study which uses cellular rejuvenation to protect retinal neurons in a mouse model of multiple sclerosis, preserving the vision of the mice.

Study reviewed:

In this manuscript, Drake et al. describe an aging-like transcriptional signature in retinal ganglion cells during experimental autoimmune encephalomyelitis (EAE) like that of cortical neurons in patients with multiple sclerosis. Partial reprogramming with AAV2-Oct4-Sox2-Kl4 to rejuvenate the transcriptome results in improved RGC survival and visual acuity during EAE.

In this edition, we’ll take a look at a Canadian study which shows that reducing a worm’s ability to fight free radicals in a specific organ could increase it’s lifespan. Does this have any implications for humans?

Contents:

Intro 0:00
Graphical Abstract 1:49
Figure 1. Tissue-specific re-expression of sod-2 rescues deficits in fertility and embryonic lethality in clk-1;sod-2 mutants 2:28
Figure 2. Tissue-specific re-expression of sod-2 can decrease stress resistance in clk-1;sod-2 worms 5:37
Figure 3. Tissue-specific re-expression of sod-2 is not sufficient to reduce clk-1;sod-2 lifespan 6:41
Figure 4. Disruption of mitochondrial superoxide dismutase in the intestine is sufficient to increase lifespan 7:33
Figure 5. Intestine-specific knockdown of sod-2 is sufficient to enhance resistance to heat stress 9:33
Figure 6. Intestine-specific knockdown of sod-2 does not affect physiologic rates 10:29
Conclusion & Next Steps 11:40

Study reviewed:
Intestine-specific disruption of mitochondrial superoxide dismutase extends longevity.
https://www.sciencedirect.com/science/article/pii/S0891584925000383

2009 paper from the same authors:
Deletion of the Mitochondrial Superoxide Dismutase sod-2 Extends Lifespan in Caenorhabditis elegans.
https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.

General overview of the pros and cons of free radicals for lifespan:

Introducing a new weekly video series showcasing the latest impactful longevity related studies.

” +Study reviewed: Reducing functionally defective old HSCs alleviates aging-related phenotypes in old recipient mice.

A new weekly series showcasing the latest and most impactful longevity studies.

In this first edition, we’ll take a look at how a subset of blood stem cells from old mice can be used to rejuvenate the blood & immune systems of other old mice and increase their lifespans.

Study reviewed:

Reviewing a trial where young blood plasma was used during joint replacement surgery in older adults, reducing immune system related inflammation and speeding up recovery. This is one of the first human trials of young plasma after many positive results in mice.

Contents:

Intro 0:00
Study Overview 1:07
Proteomic Changes 1:45
Immune Response 3:07
Specific Immune Cell Changes 4:22
Patient Outcomes 6:09
Conclusion 7:44

Study reviewed:
Infusion of young donor plasma components in older patients modifies the immune and inflammatory response to surgical tissue injury: a randomized clinical trial.
https://translational-medicine.biomedcentral.com/articles/10…45;06215-w.

If you liked this video, please consider supporting its creation via a donation to the Canadian Longevity Association, which will not only help create these videos, but also help our other advocacy and outreach efforts:
https://www.zeffy.com/en-CA/donation-form/c47dd699-b5bd-4d28…26f77ddcf3

Or, order something from our new merchandise shop:

Kicking off the first Longevity Review of 2025 with a look at how exosomes can reverse cellular senescence and lengthen lifespan; how there is a subset of youthful stem cells in older animals which can increase lifespan; the most comprehensive study of life extending molescules in the roundworm; and, in the Canadian content study, how disrupting antioxidant defences in a single organ can extend longevity. https://youtu.be/uiEcPFH0EDk

Kicking off the new year with a lifespan special, we will take a look at reversing the senescence of senescent cells to increase mouse lifespan; the discovery and beneficial effects of a subset of youthful stem cells which can also increase mouse longevity; the most comprehensive study of life-extending molecules in the roundworm c.elegans ; and in the Canadian Content study, how disrupting the antioxidant defences in a specific organ in c.elegans can increase its lifespan.

Longevity Snapshot #5 — Reviewing a trial which shows that a combination of Omega 3, Vitamin D and Exercise slows down biological aging in older adults according to 4 epigenetic clocks.

Applying epigenetic clocks to samples from the DO-HEALTH trial, Bischoff-Ferrari et al. report a small protective effect of omega-3 supplementation over 3 years on several clocks and an additive protective effect of omega-3, vitamin D and exercise using PhenoAge.